Characterization of anti-DNA B cells that escape negative selection

Editing and escape from editing in anti-DNA B cells.

Tolerance to dsDNA is achieved through editing of Ig receptors that react with dsDNA. Nevertheless, some B cells with anti-dsDNA receptors escape editing and migrate to the spleen. Certain anti-dsDNA B cells that are recovered as hybridomas from the spleens of anti-dsDNA H chain transgenic mice also bind an additional, Golgi-associated antigen. B cells that bind this antigen accumulate intracel...

Editing Anti-DNA B Cells by Vλx

Receptor editing is performed by replacement of Vkappa genes that contribute to autoreactivity. In addition, the Ckappa locus can be deleted by Vkappa rearrangement to intronic or 3' of Ckappa RS sequences (also referred to as kappa deletion elements). B cells that delete the Ckappa can then express lambda light chains. However, the lambda locus, either of man or mouse, does not allow V gene re...

Selection of anti-double-stranded DNA B cells in autoimmune MRL-lpr/lpr mice.

Abs to DNA and nucleoproteins are expressed in systemic autoimmune diseases, whereas B cells producing such Abs are edited, deleted, or inactivated in healthy individuals. Why autoimmune individuals fail to regulate is not well understood. In this study, we investigate the sources of anti-dsDNA B cells in autoimmune transgenic MRL-lpr/lpr mice. These mice are particularly susceptible to lupus b...

generation and characterization of anti-cd34 monoclonal antibodies that react with hematopoietic stem cells

cd34 is a type i membrane protein with a molecular mass of approximately 110 kda. this antigen is associated with human hematopoietic progenitor cells and is a differentiation stage-specific leukocyte antigen. in this study we have generated and characterized monoclonal antibodies (mabs) directed against a cd34 marker. mice were immunized with two keyhole lympet hemocyanin (klh)-conjugated cd34...

Differs from That of B-1a Cells The Selection of Marginal Zone B Cells